The Arkansas Department of Human Services sent the emergency revocation Friday to Alice and Ronald McClure, owners of Primary Learning Academy.
They're accused of leaving an 8-month-old alone at the end of the day. Parents say they arrived Thursday evening to pick up their baby and found the facility closed and the doors locked.
The boy was treated and cleared by EMS, police said.
"This action placed the life of the child at risk," the state's letter to the owners states.
The daycare owners were in court Friday, charged with endangering the welfare of a minor.
The McClures have 10 days to request an appeal that they must request in writing. They're set to be back in court in two weeks.